MersV1, France, Analysis, bibRecord, 000333

Single‐step nested polymerase chain reaction for detection of different genotypes of hepatitis C virus

Identifieur interne : 000333 ( France/Analysis ); précédent : 000332; suivant : 000334

Single‐step nested polymerase chain reaction for detection of different genotypes of hepatitis C virus

Auteurs : Ji-Su Li [France, États-Unis] ; Shu-Ping Tong [France, États-Unis] ; Ludmila Vitvitski [France] ; Christian Trépo [France]

Source :

Journal of Medical Virology [ 0146-6615 ] ; 1995-02.

RBID : ISTEX:92260B2629F9E5C793E467E5C939F167795C934B

Abstract

Hepatitis C virus (HCV) exhibits considerable sequence variability and circulates in the blood at extremely low levels. Current methods for detecting HCV RNA are based mostly on nested polymerase chain reaction (PCR), in which part of the first amplification product is reamplified in the second tube by an internal primer pair. A novel nested PCR method was developed in which the two successive amplification processes are carried out in the same tube with a single step of physical manipulation. Careful selection of highly conserved sequences of the 5′ noncoding region as primers enabled successful detection of all three major genotypes circulating in France, including the one with variation in this region. Retaining high sensitivity of the conventional nested PCR, the novel method reduced greatly the risk of carry‐over contaminations. It was also cost‐ and time‐saving. The one‐step nested PCR method is especially suitable for routine diagnosis of HCV infection in clinical laboratories. © 1995 Wiley‐Liss, Inc.

Url:

https://api.istex.fr/ark:/67375/WNG-67GG3H3B-J/fulltext.pdf

DOI: 10.1002/jmv.1890450207

Affiliations:

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to stream Istex, to step Corpus: 000583
to stream Istex, to step Curation: 000583
to stream Istex, to step Checkpoint: 001826
to stream Main, to step Merge: 004196
to stream Main, to step Curation: 004135
to stream Main, to step Exploration: 004135
to stream France, to step Extraction: 000333

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ISTEX:92260B2629F9E5C793E467E5C939F167795C934B

Le document en format XML

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<author><name sortKey="Vitvitski, Ludmila" sort="Vitvitski, Ludmila" uniqKey="Vitvitski L" first="Ludmila" last="Vitvitski">Ludmila Vitvitski</name>
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<front><div type="abstract" xml:lang="en">Hepatitis C virus (HCV) exhibits considerable sequence variability and circulates in the blood at extremely low levels. Current methods for detecting HCV RNA are based mostly on nested polymerase chain reaction (PCR), in which part of the first amplification product is reamplified in the second tube by an internal primer pair. A novel nested PCR method was developed in which the two successive amplification processes are carried out in the same tube with a single step of physical manipulation. Careful selection of highly conserved sequences of the 5′ noncoding region as primers enabled successful detection of all three major genotypes circulating in France, including the one with variation in this region. Retaining high sensitivity of the conventional nested PCR, the novel method reduced greatly the risk of carry‐over contaminations. It was also cost‐ and time‐saving. The one‐step nested PCR method is especially suitable for routine diagnosis of HCV infection in clinical laboratories. © 1995 Wiley‐Liss, Inc.</div>
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Single‐step nested polymerase chain reaction for detection of different genotypes of hepatitis C virus

Single‐step nested polymerase chain reaction for detection of different genotypes of hepatitis C virus

Source :

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri